› 1gz4

X-ray diffraction
2.2Å resolution

molecular mechanism of the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate

Released:
DOI: 10.2210/pdb1gz4/pdb
PDB entry 1gz4 coloured by chain, front view.
PDB entry 1gz4 coloured by chain, side view.
PDB entry 1gz4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate.
Yang Z, Lanks CW, Tong L
Structure 10 951-60 (2002)
PMID: 12121650

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent malic enzyme, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 551 amino acids
Theoretical weight: 62.55 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P23368 (Residues: 23-573; Coverage: 94%)
Gene name: ME2
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 8 x ATP
Ligand TTN 4 x TTN
Ligand MN 4 x MN
Ligand FUM 4 x FUM
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: C2
Unit cell:
a: 224.944Å b: 117.454Å c: 111.61Å
α: 90° β: 109.33° γ: 90°
R-values:
R R work R free
0.209 0.209 0.245

Quick links

Citations

5 review citations

The power of two: protein dimerization in biology.
Marianayagam et al. (2004)
4 more

3 mentions without citation

Functional Roles of Metabolic Intermediates in Regulating the Human Mitochondrial NAD(P)+-Dependent Malic Enzyme.
Hsieh et al. (2019)
2 more