› 1gz7

X-ray diffraction
1.97Å resolution

Crystal structure of the closed state of lipase 2 from Candida rugosa

Released:
DOI: 10.2210/pdb1gz7/pdb
PDB entry 1gz7 coloured by chain, front view.
PDB entry 1gz7 coloured by chain, side view.
PDB entry 1gz7 coloured by chain, top view.
Source organism: Diutina rugosa
Primary publication:
Structural insights into the lipase/esterase behavior in the Candida rugosa lipases family: crystal structure of the lipase 2 isoenzyme at 1.97A resolution.
Mancheño JM, Pernas MA, Martínez MJ, Ochoa B, Rúa ML, Hermoso JA
J Mol Biol 332 1059-69 (2003)
PMID: 14499609

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lipase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 534 amino acids
Theoretical weight: 57.51 KDa
Source organism: Diutina rugosa
UniProt:
  • Canonical: P32946 (Residues: 15-548; Coverage: 100%)
Gene name: LIP2
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P1
Unit cell:
a: 61.15Å b: 91.14Å c: 108.46Å
α: 90.78° β: 106.31° γ: 86.91°
R-values:
R R work R free
0.2 0.2 0.236

Quick links

Citations

7 review citations

Understanding Candida rugosa lipases: an overview.
Domínguez de María et al. (2006)
6 more

3 mentions without citation

Evolutionary history of versatile-lipases from Agaricales through reconstruction of ancestral structures.
Barriuso et al. (2017)
2 more