› 1gzn

X-ray diffraction
2.5Å resolution

Structure of PKB kinase domain

Released:
DOI: 10.2210/pdb1gzn/pdb
PDB entry 1gzn coloured by chain, front view.
PDB entry 1gzn coloured by chain, side view.
PDB entry 1gzn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanism for the regulation of protein kinase B/Akt by hydrophobic motif phosphorylation.
Yang J, Cron P, Thompson V, Good VM, Hess D, Hemmings BA, Barford D
Mol Cell 9 1227-40 (2002)
PMID: 12086620

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RAC-beta serine/threonine-protein kinase Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 38.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P31751 (Residues: 146-480; Coverage: 70%)
Gene name: AKT2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 149.523Å b: 149.523Å c: 39.055Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.268 0.319
Expression system: Spodoptera frugiperda

Quick links

Citations

62 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
61 more

14 mentions without citation

Signaling through cAMP and cAMP-dependent protein kinase: diverse strategies for drug design.
Taylor et al. (2008)
13 more