› 1h0a

X-ray diffraction
1.7Å resolution

Epsin ENTH bound to Ins(1,4,5)P3

Released:
DOI: 10.2210/pdb1h0a/pdb
PDB entry 1h0a coloured by chain, front view.
PDB entry 1h0a coloured by chain, side view.
PDB entry 1h0a coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Curvature of clathrin-coated pits driven by epsin.
Ford MG, Mills IG, Peter BJ, Vallis Y, Praefcke GJ, Evans PR, McMahon HT
Nature 419 361-6 (2002)
PMID: 12353027

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epsin-1 Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 18.42 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O88339 (Residues: 1-158; Coverage: 28%)
Gene name: Epn1
Sequence domains: ENTH domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

2 bound ligands:
Ligand DIO 5 x DIO
Ligand I3P 1 x I3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 41.209Å b: 95.823Å c: 118.151Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.185 0.228
Expression system: Escherichia coli BL21

Quick links

Citations

193 review citations

Regulated portals of entry into the cell.
Conner et al. (2003)
192 more

27 mentions without citation

Molecular structure, function, and dynamics of clathrin-mediated membrane traffic.
Kirchhausen et al. (2014)
26 more