› 1h0b

X-ray diffraction
1.8Å resolution

Endoglucanase cel12A from Rhodothermus marinus

Released:
DOI: 10.2210/pdb1h0b/pdb
PDB entry 1h0b coloured by chain, front view.
PDB entry 1h0b coloured by chain, side view.
PDB entry 1h0b coloured by chain, top view.
Source organism: Rhodothermus marinus
Primary publication:
The structure of Rhodothermus marinus Cel12A, a highly thermostable family 12 endoglucanase, at 1.8 A resolution.
Crennell SJ, Hreggvidsson GO, Nordberg Karlsson E
J Mol Biol 320 883-97 (2002)
PMID: 12095262

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulase Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 28.5 KDa
Source organism: Rhodothermus marinus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O33897 (Residues: 38-126, 127-260; Coverage: 86%)
Gene name: celA
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 56.099Å b: 67.779Å c: 132.262Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.194
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Thermostable enzymes as biocatalysts in the biofuel industry.
Yeoman et al. (2010)
2 more

6 mentions without citation

A highly acid-stable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus.
Huang et al. (2005)
5 more