› 1h1l

X-ray diffraction
1.9Å resolution

NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT

Released:
DOI: 10.2210/pdb1h1l/pdb
PDB entry 1h1l coloured by chain, front view.
PDB entry 1h1l coloured by chain, side view.
PDB entry 1h1l coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco).
Mayer SM, Gormal CA, Smith BE, Lawson DM
J Biol Chem 277 35263-6 (2002)
PMID: 12133839

Function and Biology Details

Reaction catalysed: 
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132542 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nitrogenase molybdenum-iron protein alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 481 amino acids
Theoretical weight: 53.92 KDa
Source organism: Klebsiella pneumoniae
UniProt:
  • Canonical: P00466 (Residues: 3-483; Coverage: 100%)
Gene name: nifD
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Structure domains: Nitrogenase molybdenum iron protein domain
Nitrogenase molybdenum-iron protein beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 519 amino acids
Theoretical weight: 58.34 KDa
Source organism: Klebsiella pneumoniae
UniProt:
  • Canonical: P09772 (Residues: 2-520; Coverage: 100%)
Gene name: nifK
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CIT 2 x CIT
Ligand CFM 2 x CFM
Ligand MG 4 x MG
Ligand CLF 2 x CLF
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 204.06Å b: 75.06Å c: 164.26Å
α: 90° β: 123.95° γ: 90°
R-values:
R R work R free
0.176 0.176 0.236

Quick links

Citations

6 review citations

Mechanism of Mo-dependent nitrogenase.
Seefeldt et al. (2009)
5 more

9 mentions without citation

A Structural-informatics approach for tracing beta-sheets: building pseudo-C(alpha) traces for beta-strands in intermediate-resolution density maps.
Kong et al. (2004)
8 more