› 1h2f

X-ray diffraction
2Å resolution

BACILLUS STEAROTHERMOPHILUS PHOE (previously known as yhfr) in complex with trivanadate

Released:
DOI: 10.2210/pdb1h2f/pdb
PDB entry 1h2f coloured by chain, front view.
PDB entry 1h2f coloured by chain, side view.
PDB entry 1h2f coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structures of phosphate and trivanadate complexes of Bacillus stearothermophilus phosphatase PhoE: structural and functional analysis in the cofactor-dependent phosphoglycerate mutase superfamily.
Rigden DJ, Littlejohn JE, Henderson K, Jedrzejas MJ
J. Mol. Biol. 325 411-20 (2003)
PMID: 12498792

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycerate mutase Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.65 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ALU0 (Residues: 1-195; Coverage: 100%)
Gene name: yhfR
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

2 bound ligands:
Ligand VA3 1 x VA3
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 55.758Å b: 55.758Å c: 163.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The power of vanadate in crystallographic investigations of phosphoryl transfer enzymes.
Davies et al. (2004)
1 more

4 mentions without citation

Unique attributes of cyanobacterial metabolism revealed by improved genome-scale metabolic modeling and essential gene analysis.
Broddrick et al. (2016)
3 more