› 1h2j

X-ray diffraction
1.15Å resolution

ENDOGLUCANASE CEL5A IN COMPLEX WITH UNHYDROLYSED AND COVALENTLY LINKED 2,4-DINITROPHENYL-2-DEOXY-2-FLUORO-CELLOBIOSIDE AT 1.15 A RESOLUTION

Released:
DOI: 10.2210/pdb1h2j/pdb
PDB entry 1h2j coloured by chain, front view.
PDB entry 1h2j coloured by chain, side view.
PDB entry 1h2j coloured by chain, top view.
Source organism: Salipaludibacillus agaradhaerens
Primary publication:
Direct experimental observation of the hydrogen-bonding network of a glycosidase along its reaction coordinate revealed by atomic resolution analyses of endoglucanase Cel5A.
Varrot A, Davies GJ
Acta Crystallogr D Biol Crystallogr 59 447-52 (2003)
PMID: 12595701

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase 5A Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 34 KDa
Source organism: Salipaludibacillus agaradhaerens
Expression system: Bacillus subtilis
UniProt:
  • Canonical: O85465 (Residues: 27-329; Coverage: 81%)
Gene name: cel5A
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand DCB 1 x DCB
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 54.355Å b: 69.572Å c: 77.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.118 0.117 0.136
Expression system: Bacillus subtilis

Quick links

Citations

1 review citation

Understanding nature's catalytic toolkit.
Gutteridge et al. (2005)
11 other articles

5 mentions without citation

Crystal structure and snapshots along the reaction pathway of a family 51 alpha-L-arabinofuranosidase.
Hövel et al. (2003)
4 more