› 1h3d

X-ray diffraction
2.7Å resolution

STRUCTURE OF THE E.COLI ATP-PHOSPHORIBOSYLTRANSFERASE

Released:
DOI: 10.2210/pdb1h3d/pdb
PDB entry 1h3d coloured by chain, front view.
PDB entry 1h3d coloured by chain, side view.
PDB entry 1h3d coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The structure of Escherichia coli ATP-phosphoribosyltransferase: identification of substrate binding sites and mode of AMP inhibition.
Lohkamp B, McDermott G, Campbell SA, Coggins JR, Lapthorn AJ
J Mol Biol 336 131-44 (2004)
PMID: 14741209

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 33.41 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P60757 (Residues: 1-299; Coverage: 100%)
Gene names: JW2001, b2019, hisG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AMP 1 x AMP
Ligand TLA 2 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: R32
Unit cell:
a: 133.645Å b: 133.645Å c: 114.118Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.222 0.277
Expression system: Escherichia coli

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Citations

7 review citations

Cyclic di-AMP: another second messenger enters the fray.
Corrigan et al. (2013)
6 more

3 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
2 more