› 1h3g

X-ray diffraction
2.1Å resolution

Cyclomaltodextrinase from Flavobacterium sp. No. 92: from DNA sequence to protein structure

Released:
DOI: 10.2210/pdb1h3g/pdb
PDB entry 1h3g coloured by chain, front view.
PDB entry 1h3g coloured by chain, side view.
PDB entry 1h3g coloured by chain, top view.
Source organism: Flavobacterium sp. 92
Primary publication:
Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92.
Fritzsche HB, Schwede T, Schulz GE
Eur. J. Biochem. 270 2332-41 (2003)
PMID: 12752453

Function and Biology Details

Reaction catalysed: 
Cyclomaltodextrin + H(2)O = linear maltodextrin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 601 amino acids
Theoretical weight: 69.26 KDa
Source organism: Flavobacterium sp. 92
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KKG0 (Residues: 24-619; Coverage: 99%)
Gene name: cdase
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand MSE 50 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: R32
Unit cell:
a: 181.339Å b: 181.339Å c: 231.511Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.188 0.223
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The Sus operon: a model system for starch uptake by the human gut Bacteroidetes.
Foley et al. (2016)
17 other articles

3 mentions without citation

Structural investigation of the thermostability and product specificity of amylosucrase from the bacterium Deinococcus geothermalis.
Guérin et al. (2012)
2 more