› 1h41

X-ray diffraction
1.5Å resolution

Pseudomonas cellulosa E292A alpha-D-glucuronidase mutant complexed with aldotriuronic acid

Released:
DOI: 10.2210/pdb1h41/pdb
PDB entry 1h41 coloured by chain, front view.
PDB entry 1h41 coloured by chain, side view.
PDB entry 1h41 coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
The alpha-glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants.
Nagy T, Nurizzo D, Davies GJ, Biely P, Lakey JH, Bolam DN, Gilbert HJ
J Biol Chem 278 20286-92 (2003)
PMID: 12654910

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->2)-alpha-D-(4-O-methyl)glucuronosyl links in the main chain of hardwood xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular xylan exo-alpha-(1->2)-glucuronosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 708 amino acids
Theoretical weight: 80.38 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli
UniProt:
  • Canonical: B3PC73 (Residues: 25-732; Coverage: 99%)
Gene names: CJA_2887, gla67A, glcA67A
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GCV 2 x GCV
Ligand EDO 28 x EDO
Ligand CO 10 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P1
Unit cell:
a: 69.412Å b: 74.393Å c: 87.462Å
α: 115.09° β: 93.04° γ: 109.44°
R-values:
R R work R free
0.118 0.118 0.153
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus.
Gardner JG. (2016)
1 more

1 mention without citation

Protein 3D Structure and Electron Microscopy Map Retrieval Using 3D-SURFER2.0 and EM-SURFER.
Han et al. (2017)