› 1h4h

X-ray diffraction
1.9Å resolution

Oligosaccharide-binding to family 11 xylanases: both covalent intermediate and mutant-product complexes display 2,5B conformations at the active-centre

Released:
DOI: 10.2210/pdb1h4h/pdb
PDB entry 1h4h coloured by chain, front view.
PDB entry 1h4h coloured by chain, side view.
PDB entry 1h4h coloured by chain, top view.
Source organism: Salipaludibacillus agaradhaerens
Primary publication:
Catalysis and specificity in enzymatic glycoside hydrolysis: a 2,5B conformation for the glycosyl-enzyme intermediate revealed by the structure of the Bacillus agaradhaerens family 11 xylanase.
Sabini E, Sulzenbacher G, Dauter M, Dauter Z, Jørgensen PL, Schülein M, Dupont C, Davies GJ, Wilson KS
Chem Biol 6 483-92 (1999)
PMID: 10381409

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181965 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-1,4-beta-xylanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 209 amino acids
Theoretical weight: 23.31 KDa
Source organism: Salipaludibacillus agaradhaerens
Expression system: Bacillus licheniformis
UniProt:
  • Canonical: Q7SIE2 (Residues: 1-209; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: XYS, XYP
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 74.344Å b: 78.895Å c: 76.273Å
α: 90° β: 91.93° γ: 90°
R-values:
R R work R free
0.184 0.184 0.241
Expression system: Bacillus licheniformis

Quick links

Citations

5 review citations

Glycosidase mechanisms.
Rye et al. (2000)
4 more

1 mention without citation

Structural analysis of a glycoside hydrolase family 11 xylanase from Neocallimastix patriciarum: insights into the molecular basis of a thermophilic enzyme.
Cheng et al. (2014)