› 1h4j

X-ray diffraction
3Å resolution

Function and Biology Details

Reaction catalysed: 
A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L) + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147076 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methanol dehydrogenase [cytochrome c] subunit 1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 599 amino acids
Theoretical weight: 65.88 KDa
Source organism: Methylorubrum extorquens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16027 (Residues: 28-626; Coverage: 100%)
Gene names: MexAM1_META1p4538, moxF, mxaF
Sequence domains:
Structure domains: Quinoprotein alcohol dehydrogenase-like superfamily
Methanol dehydrogenase [cytochrome c] subunit 2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 74 amino acids
Theoretical weight: 8.48 KDa
Source organism: Methylorubrum extorquens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14775 (Residues: 23-96; Coverage: 100%)
Gene names: MexAM1_META1p4535, moxI, mxaI
Sequence domains: Methanol dehydrogenase beta subunit
Structure domains: Methanol dehydrogenase, beta subunit

Ligands and Environments


Cofactor: Ligand PQQ 4 x PQQ
1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P21
Unit cell:
a: 101.85Å b: 61.02Å c: 212.68Å
α: 90° β: 92.83° γ: 90°
R-values:
R R work R free
0.187 0.187 0.217
Expression system: Escherichia coli

Quick links

Citations

5 review citations

PQQ-dependent methanol dehydrogenases: rare-earth elements make a difference.
Keltjens et al. (2014)
4 more

1 mention without citation

Understanding the impacts of missense mutations on structures and functions of human cancer-related genes: A preliminary computational analysis of the COSMIC Cancer Gene Census.
Malhotra et al. (2019)