› 1h4x

X-ray diffraction
1.16Å resolution

Structure of the Bacillus Cell Fate Determinant SpoIIAA in the Phosphorylated Form

Released:
DOI: 10.2210/pdb1h4x/pdb
PDB entry 1h4x coloured by chain, front view.
PDB entry 1h4x coloured by chain, side view.
PDB entry 1h4x coloured by chain, top view.
Source organism: Lysinibacillus sphaericus
Primary publication:
Structure of the Bacillus cell fate determinant SpoIIAA in phosphorylated and unphosphorylated forms.
Seavers PR, Lewis RJ, Brannigan JA, Verschueren KH, Murshudov GN, Wilkinson AJ
Structure 9 605-14 (2001)
PMID: 11470435

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anti-sigma F factor antagonist Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 13.21 KDa
Source organism: Lysinibacillus sphaericus
Expression system: Escherichia coli
UniProt:
  • Canonical: O32723 (Residues: 1-117; Coverage: 100%)
Gene name: spoIIAA
Sequence domains: STAS domain
Structure domains: STAS domain

Ligands and Environments

1 bound ligand:
Ligand TRS 2 x TRS
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 51.119Å b: 61.577Å c: 65.892Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.133 0.164
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Stress responses of bacteria.
Marles-Wright et al. (2007)
3 more

10 mentions without citation

The importance of intrinsic disorder for protein phosphorylation.
Iakoucheva et al. (2004)
9 more