› 1h5s

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153475 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Glucose-1-phosphate thymidylyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 32.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37744 (Residues: 1-293; Coverage: 100%)
Gene names: JW2024, b2039, rfbA, rmlA, rmlA1
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
Glucose-1-phosphate thymidylyltransferase 1 Chain: B
Molecule details ›
Chain: B
Length: 293 amino acids
Theoretical weight: 32.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37744 (Residues: 1-293; Coverage: 100%)
Gene names: JW2024, b2039, rfbA, rmlA, rmlA1
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
Glucose-1-phosphate thymidylyltransferase 1 Chain: C
Molecule details ›
Chain: C
Length: 293 amino acids
Theoretical weight: 32.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37744 (Residues: 1-293; Coverage: 100%)
Gene names: JW2024, b2039, rfbA, rmlA, rmlA1
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
Glucose-1-phosphate thymidylyltransferase 1 Chain: D
Molecule details ›
Chain: D
Length: 293 amino acids
Theoretical weight: 32.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37744 (Residues: 1-293; Coverage: 100%)
Gene names: JW2024, b2039, rfbA, rmlA, rmlA1
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

1 bound ligand:
Ligand TMP 8 x TMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21
Unit cell:
a: 73.1Å b: 119.27Å c: 81.11Å
α: 90° β: 112.66° γ: 90°
R-values:
R R work R free
0.176 0.176 0.257
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly.
Samuel et al. (2003)
6 more

3 mentions without citation

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
2 more