› 1h74

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF HOMOSERINE KINASE COMPLEXED WITH ILE

Released:
DOI: 10.2210/pdb1h74/pdb
PDB entry 1h74 coloured by chain, front view.
PDB entry 1h74 coloured by chain, side view.
PDB entry 1h74 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural basis for the catalysis and substrate specificity of homoserine kinase.
Krishna SS, Zhou T, Daugherty M, Osterman A, Zhang H
Biochemistry 40 10810-8 (2001)
PMID: 11535056

Function and Biology Details

Reaction catalysed: 
ATP + L-homoserine = ADP + O-phospho-L-homoserine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homoserine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 296 amino acids
Theoretical weight: 32.3 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58504 (Residues: 1-296; Coverage: 100%)
Gene names: MJ1104, thrB
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ILE 4 x ILE
Ligand ADP 2 x ADP
Ligand AGS 2 x AGS
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 61.993Å b: 128.721Å c: 109.423Å
α: 90° β: 105.57° γ: 90°
R-values:
R R work R free
0.21 0.21 0.238
Expression system: Escherichia coli

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Citations

5 review citations

Structure and function of enzymes of the Leloir pathway for galactose metabolism.
Holden et al. (2003)
4 more

2 mentions without citation

Helitron distribution in Brassicaceae and whole Genome Helitron density as a character for distinguishing plant species.
Hu et al. (2019)
1 more