› 1h7g

X-ray diffraction
2.13Å resolution

The structure of CMP:2-keto-3-deoxy-manno-octonic acid synthetase and of its complexes with substrates and substrate analogues, CTP MG2+ complex

Released:
DOI: 10.2210/pdb1h7g/pdb
PDB entry 1h7g coloured by chain, front view.
PDB entry 1h7g coloured by chain, side view.
PDB entry 1h7g coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of CMP:2-keto-3-deoxy-manno-octonic acid synthetase and of its complexes with substrates and substrate analogs.
Jelakovic S, Schulz GE
J Mol Biol 312 143-55 (2001)
PMID: 11545592

Function and Biology Details

Reaction catalysed: 
CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-deoxy-manno-octulosonate cytidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P42216 (Residues: 2-246; Coverage: 100%)
Gene name: kpsU
Sequence domains: Cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand CTP 1 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 46.04Å b: 133.58Å c: 48.41Å
α: 90° β: 102.45° γ: 90°
R-values:
R R work R free
0.175 0.175 0.232
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Natural-product sugar biosynthesis and enzymatic glycodiversification.
Thibodeaux et al. (2008)
2 more

1 mention without citation

Evidence for a two-metal-ion mechanism in the cytidyltransferase KdsB, an enzyme involved in lipopolysaccharide biosynthesis.
Schmidt et al. (2011)