› 1h7l

X-ray diffraction
1.98Å resolution

dTDP-MAGNESIUM COMPLEX OF SPSA FROM BACILLUS SUBTILIS

Released:
DOI: 10.2210/pdb1h7l/pdb
PDB entry 1h7l coloured by chain, front view.
PDB entry 1h7l coloured by chain, side view.
PDB entry 1h7l coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Three-dimensional structures of the Mn and Mg dTDP complexes of the family GT-2 glycosyltransferase SpsA: a comparison with related NDP-sugar glycosyltransferases.
Tarbouriech N, Charnock SJ, Davies GJ
J Mol Biol 314 655-61 (2001)
PMID: 11733986

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spore coat polysaccharide biosynthesis protein SpsA Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 30.09 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P39621 (Residues: 2-256; Coverage: 100%)
Gene names: BSU37910, ipa-63d, spsA
Sequence domains: Glycosyl transferase family 2
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand TYD 1 x TYD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 47.32Å b: 142.31Å c: 81.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.228
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
10 more

6 mentions without citation

Understanding substrate selectivity of human UDP-glucuronosyltransferases through QSAR modeling and analysis of homologous enzymes.
Dong et al. (2012)
5 more