› 1h7o

X-ray diffraction
1.75Å resolution

SCHIFF-BASE COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE WITH 5-AMINOLAEVULINIC ACID AT 1.7 A RESOLUTION

Released:
DOI: 10.2210/pdb1h7o/pdb
PDB entry 1h7o coloured by chain, front view.
PDB entry 1h7o coloured by chain, side view.
PDB entry 1h7o coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The x-ray structure of yeast 5-aminolaevulinic acid dehydratase complexed with substrate and three inhibitors.
Erskine PT, Newbold R, Brindley AA, Wood SP, Shoolingin-Jordan PM, Warren MJ, Cooper JB
J Mol Biol 312 133-41 (2001)
PMID: 11545591

Function and Biology Details

Reaction catalysed: 
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-aminolevulinic acid dehydratase Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 37.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P05373 (Residues: 1-341; Coverage: 100%)
Gene names: HEM2, YGL040C
Sequence domains: Delta-aminolevulinic acid dehydratase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand DAV 1 x DAV
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: I422
Unit cell:
a: 102.8Å b: 102.8Å c: 168.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 not available 0.353
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Human hereditary hepatic porphyrias.
Nordmann et al. (2002)
1 more