› 1h9c

Solution NMR

NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.

Released:
DOI: 10.2210/pdb1h9c/pdb
PDB entry 1h9c coloured by chain, ensemble of 9 models, front view.
PDB entry 1h9c coloured by chain, ensemble of 9 models, side view.
PDB entry 1h9c coloured by chain, ensemble of 9 models, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Primary publication:
NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.
Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM
J Mol Biol 308 993-1009 (2001)
PMID: 11352587

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + N,N'-diacetylchitobiose(Side 1) = [protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system N,N'-diacetylchitobiose-specific EIIB component Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.52 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: P69795 (Residues: 1-106; Coverage: 100%)
Gene names: JW1727, b1738, celA, chbB
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSP 1 x CSP

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY, RESTRAINED MOLECULAR DYNAMICS, SIMULATED ANNEALING
Expression system: Escherichia coli str. K-12 substr. W3110

Quick links

Citations

3 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
2 more

5 mentions without citation

Structural insight into the PTS sugar transporter EIIC.
McCoy et al. (2015)
4 more