› 1h9t

X-ray diffraction
3.25Å resolution

FADR, FATTY ACID RESPONSIVE TRANSCRIPTION FACTOR FROM E. COLI IN COMPLEX WITH FADB OPERATOR

Released:
DOI: 10.2210/pdb1h9t/pdb
PDB entry 1h9t coloured by chain, front view.
PDB entry 1h9t coloured by chain, side view.
PDB entry 1h9t coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structural basis of acyl coenzyme A-dependent regulation of the transcription factor FadR.
van Aalten DM, DiRusso CC, Knudsen J
EMBO J 20 2041-50 (2001)
PMID: 11296236

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113889 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Fatty acid metabolism regulator protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V6 (Residues: 1-239; Coverage: 100%)
Gene names: JW1176, b1187, fadR, oleR, thdB
Sequence domains:
Structure domains:
5'-D(*CP*AP*TP*CP*TP*GP*GP*TP*AP*CP*GP*AP* CP*CP*AP*GP*AP*TP*C)-3' Chain: X
Molecule details ›
Chain: X
Length: 19 nucleotides
Theoretical weight: 5.79 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*GP*AP*TP*CP*TP*GP*GP*TP*CP*GP*TP*AP* CP*CP*AP*GP*AP*TP*G)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 19 nucleotides
Theoretical weight: 5.86 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand AU 2 x AU
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P6122
Unit cell:
a: 92.992Å b: 92.992Å c: 334.826Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.264 0.32
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

Bacterial transcriptional regulators for degradation pathways of aromatic compounds.
Tropel et al. (2004)
17 more

17 mentions without citation

The structural basis of acyl coenzyme A-dependent regulation of the transcription factor FadR.
van Aalten et al. (2001)
16 more