› 1haz

X-ray diffraction
1.4Å resolution

Snapshots of serine protease catalysis: (C) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 9 for 1 minute

Released:
DOI: 10.2210/pdb1haz/pdb
PDB entry 1haz coloured by chain, front view.
PDB entry 1haz coloured by chain, side view.
PDB entry 1haz coloured by chain, top view.
Source organisms:
Primary publication:
X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate.
Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J
Nat Struct Biol 8 689-94 (2001)
PMID: 11473259

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133459 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BETA-CASOMORPHIN-7 Chain: A
Molecule details ›
Chain: A
Length: 4 amino acids
Theoretical weight: 459 Da
Source organism: Homo sapiens
Expression system: Not provided
Chymotrypsin-like elastase family member 1 Chain: B
Molecule details ›
Chain: B
Length: 240 amino acids
Theoretical weight: 25.93 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 50.57Å b: 57.67Å c: 74.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.202
Expression system: Not provided

Quick links

Citations

7 review citations

The protein structures that shape caspase activity, specificity, activation and inhibition.
Fuentes-Prior et al. (2004)
6 more

5 mentions without citation

Bioinformatics perspective on rhomboid intramembrane protease evolution and function.
Kinch et al. (2013)
4 more