› 1hdf

X-ray diffraction
2.35Å resolution

Evolution of the eye lens beta-gamma-crystallin domain fold

Released:
DOI: 10.2210/pdb1hdf/pdb
PDB entry 1hdf coloured by chain, front view.
PDB entry 1hdf coloured by chain, side view.
PDB entry 1hdf coloured by chain, top view.
Source organism: Physarum polycephalum
Primary publication:
Crystal structure of the calcium-loaded spherulin 3a dimer sheds light on the evolution of the eye lens betagamma-crystallin domain fold.
Clout NJ, Kretschmar M, Jaenicke R, Slingsby C
Structure 9 115-24 (2001)
PMID: 11250196

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spherulin-3A Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 11.23 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli
UniProt:
  • Canonical: P09353 (Residues: 2-103; Coverage: 99%)
Sequence domains: Beta/Gamma crystallin
Structure domains: Crystallins

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P43212
Unit cell:
a: 41.35Å b: 41.35Å c: 213.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 not available
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Ageing and vision: structure, stability and function of lens crystallins.
Bloemendal et al. (2004)
5 more

4 mentions without citation

Evolution of crystallins for a role in the vertebrate eye lens.
Slingsby et al. (2013)
3 more