› 1he4

X-ray diffraction
1.4Å resolution

Human biliverdin IX beta reductase: NADP/FMN ternary complex

Released:
DOI: 10.2210/pdb1he4/pdb
PDB entry 1he4 coloured by chain, front view.
PDB entry 1he4 coloured by chain, side view.
PDB entry 1he4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human biliverdin IXbeta reductase, an early fetal bilirubin IXbeta producing enzyme.
Pereira PJ, Macedo-Ribeiro S, Párraga A, Pérez-Luque R, Cunningham O, Darcy K, Mantle TJ, Coll M
Nat Struct Biol 8 215-20 (2001)
PMID: 11224564

Function and Biology Details

Reactions catalysed: 
Bilirubin + NAD(P)(+) = biliverdin + NAD(P)H
Reduced riboflavin + NADP(+) = riboflavin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin reductase (NADPH) Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30043 (Residues: 1-206; Coverage: 100%)
Gene names: BLVRB, FLR, SCAN
Sequence domains: NAD(P)H-binding
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN

Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 40.1Å b: 49.3Å c: 106.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 not available 0.18
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
11 more

5 mentions without citation

Structure of the membrane proximal oxidoreductase domain of human Steap3, the dominant ferrireductase of the erythroid transferrin cycle.
Sendamarai et al. (2008)
4 more