› 1hg3

X-ray diffraction
2.7Å resolution

Crystal structure of tetrameric TIM from Pyrococcus woesei.

Released:
DOI: 10.2210/pdb1hg3/pdb
PDB entry 1hg3 coloured by chain, front view.
PDB entry 1hg3 coloured by chain, side view.
PDB entry 1hg3 coloured by chain, top view.
Source organism: Pyrococcus woesei
Primary publication:
Tiny TIM: a small, tetrameric, hyperthermostable triosephosphate isomerase.
Walden H, Bell GS, Russell RJ, Siebers B, Hensel R, Taylor GL
J Mol Biol 306 745-57 (2001)
PMID: 11243785

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 225 amino acids
Theoretical weight: 23.72 KDa
Source organism: Pyrococcus woesei
Expression system: Escherichia coli
UniProt:
  • Canonical: P62003 (Residues: 1-225; Coverage: 99%)
Gene names: tpi, tpiA
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand 3PP 8 x 3PP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21
Unit cell:
a: 79Å b: 89.1Å c: 145.1Å
α: 90° β: 92.8° γ: 90°
R-values:
R R work R free
0.254 0.254 0.286
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Rational engineering of enzyme stability.
Eijsink et al. (2004)
9 more

11 mentions without citation

Structural insights into the mechanism of the PLP synthase holoenzyme from Thermotoga maritima.
Zein et al. (2006)
10 more