1hht

X-ray diffraction
2.9Å resolution

RNA dependent RNA polymerase from dsRNA bacteriophage phi6 plus template

Released:
Source organism: Cystovirus phi6

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113898 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
RNA-directed RNA polymerase Chains: P, Q, R
Molecule details ›
Chains: P, Q, R
Length: 664 amino acids
Theoretical weight: 74.9 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11124 (Residues: 2-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:
DNA (5'-(*TP*TP*TP*CP*C)-3') Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 5 nucleotides
Theoretical weight: 1.45 KDa
Source organism: Cystovirus phi6
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 105.089Å b: 92.239Å c: 140.64Å
α: 90° β: 101.04° γ: 90°
R-values:
R R work R free
0.237 0.237 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided