› 1hi8

X-ray diffraction
2.5Å resolution

RNA dependent RNA polymerase from dsRNA bacteriophage phi6

Released:
DOI: 10.2210/pdb1hi8/pdb
PDB entry 1hi8 coloured by chain, front view.
PDB entry 1hi8 coloured by chain, side view.
PDB entry 1hi8 coloured by chain, top view.
Source organism: Pseudomonas virus phi6
Primary publication:
A mechanism for initiating RNA-dependent RNA polymerization.
Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI
Nature 410 235-40 (2001)
PMID: 11242087

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 664 amino acids
Theoretical weight: 76.08 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11124 (Residues: 2-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 50 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P32
Unit cell:
a: 110Å b: 110Å c: 159.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.28 0.28 0.316
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

68 review citations

Replication of hepatitis C virus.
Moradpour et al. (2007)
67 more

6 mentions without citation

Cystoviral RNA-directed RNA polymerases: Regulation of RNA synthesis on multiple time and length scales.
Alphonse et al. (2017)
5 more