› 1hj3

X-ray diffraction
1.6Å resolution

Cytochrome cd1 Nitrite Reductase, dioxygen complex

Released:
DOI: 10.2210/pdb1hj3/pdb
PDB entry 1hj3 coloured by chain, front view.
PDB entry 1hj3 coloured by chain, side view.
PDB entry 1hj3 coloured by chain, top view.
Source organism: Paracoccus pantotrophus
Primary publication:
Structure of the bound dioxygen species in the cytochrome oxidase reaction of cytochrome cd1 nitrite reductase.
Sjögren T, Hajdu J
J Biol Chem 276 13072-6 (2001)
PMID: 11278884

Function and Biology Details

Reactions catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
NH(3) + H(2)O + acceptor = hydroxylamine + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrite reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 567 amino acids
Theoretical weight: 62.55 KDa
Source organism: Paracoccus pantotrophus
UniProt:
  • Canonical: P72181 (Residues: 30-596; Coverage: 100%)
Gene name: nirS
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand DHE 2 x DHE

Cofactor: Ligand HEC 2 x HEC
3 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 3 x SO4
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 107.19Å b: 61.17Å c: 100.52Å
α: 90° β: 112.19° γ: 90°
R-values:
R R work R free
0.196 0.196 0.219

Quick links

Citations

3 review citations

Advances in kinetic protein crystallography.
Bourgeois et al. (2005)
2 more

1 mention without citation

Protein subunit interfaces: heterodimers versus homodimers.
Zhanhua et al. (2005)