› 1hjs

X-ray diffraction
1.87Å resolution

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.

Released:
DOI: 10.2210/pdb1hjs/pdb
PDB entry 1hjs coloured by chain, front view.
PDB entry 1hjs coloured by chain, side view.
PDB entry 1hjs coloured by chain, top view.
Source organism: Thermothelomyces thermophilus
Primary publication:
Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.
Le Nours J, Ryttersgaard C, Lo Leggio L, Østergaard PR, Borchert TV, Christensen LL, Larsen S
Protein Sci 12 1195-204 (2003)
PMID: 12761390

Function and Biology Details

Reaction catalysed: 
The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160721 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arabinogalactan endo-beta-1,4-galactanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 332 amino acids
Theoretical weight: 36.84 KDa
Source organism: Thermothelomyces thermophilus
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P83692 (Residues: 1-332; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 53
Structure domains: Glycosidases

Ligands and Environments

4 bound ligands:
Ligand NAG 4 x NAG
Ligand SO4 11 x SO4
Ligand PEG 13 x PEG
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2
Unit cell:
a: 108.375Å b: 136.145Å c: 111.007Å
α: 90° β: 119.02° γ: 90°
R-values:
R R work R free
0.194 0.194 0.208
Expression system: Aspergillus oryzae

Quick links

Citations

2 review citations

Myceliophthora thermophila syn. Sporotrichum thermophile: a thermophilic mould of biotechnological potential.
Singh B. (2016)
1 more

5 mentions without citation

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.
Le Nours et al. (2003)
4 more