› 1hk9

X-ray diffraction
2.15Å resolution

Crystal structure of the Hfq protein from Escherichia coli

Released:
DOI: 10.2210/pdb1hk9/pdb
PDB entry 1hk9 coloured by chain, front view.
PDB entry 1hk9 coloured by chain, side view.
PDB entry 1hk9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli.
Sauter C, Basquin J, Suck D
Nucleic Acids Res 31 4091-8 (2003)
PMID: 12853626

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 74 amino acids
Theoretical weight: 8.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A6X3 (Residues: 1-72; Coverage: 71%)
Gene names: JW4130, b4172, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P61
Unit cell:
a: 61.35Å b: 61.35Å c: 166.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.262
Expression system: Escherichia coli BL21

Quick links

Citations

26 review citations

The small RNA regulators of Escherichia coli: roles and mechanisms*.
Gottesman S. (2004)
25 more

37 mentions without citation

The Escherichia Coli Hfq Protein: An Unattended DNA-Transactions Regulator.
Cech et al. (2016)
36 more