› 1hkh

X-ray diffraction
1.73Å resolution

unligated gamma lactamase from an Aureobacterium species

Released:
DOI: 10.2210/pdb1hkh/pdb
PDB entry 1hkh coloured by chain, front view.
PDB entry 1hkh coloured by chain, side view.
PDB entry 1hkh coloured by chain, top view.
Source organism: Microbacterium
Primary publication:
The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site.
Line K, Isupov MN, Littlechild JA
J Mol Biol 338 519-32 (2004)
PMID: 15081810

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 30.88 KDa
Source organism: Microbacterium
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q8GJP7 (Residues: 2-280; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: F23
Unit cell:
a: 240.874Å b: 240.874Å c: 240.874Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.144 0.158
Expression system: Escherichia coli DH5[alpha]

Quick links

Citations

2 review citations

Scope, limitations and classification of lactamases.
Assaf et al. (2016)
1 more

4 mentions without citation

Enzymes from Extreme Environments and Their Industrial Applications.
Littlechild JA. (2015)
3 more