› 1hkq

X-ray diffraction
2.75Å resolution

PPS10 plasmid DNA replication initiator protein RepA. Replication inactive, dimeric N-terminal domain.

Released:
DOI: 10.2210/pdb1hkq/pdb
PDB entry 1hkq coloured by chain, front view.
PDB entry 1hkq coloured by chain, side view.
PDB entry 1hkq coloured by chain, top view.
Source organism: Pseudomonas savastanoi
Primary publication:
Remodeling of replication initiator proteins.
Forest KT, Filutowicz MS
Nat Struct Biol 10 496-8 (2003)
PMID: 12825085

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Initiator Rep protein WH1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.18 KDa
Source organism: Pseudomonas savastanoi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q52546 (Residues: 2-133; Coverage: 57%)
Gene name: repA
Sequence domains: Initiator Replication protein, WH1
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

3 bound ligands:
Ligand HG 8 x HG
Ligand BEZ 2 x BEZ
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P212121
Unit cell:
a: 51.866Å b: 55.533Å c: 96.073Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.272
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Role of pi dimers in coupling ("handcuffing") of plasmid R6K's gamma ori iterons.
Kunnimalaiyaan et al. (2005)
3 more

10 mentions without citation

Amyloidogenic sequences in native protein structures.
Tzotzos et al. (2010)
9 more