› 1hku

X-ray diffraction
2.3Å resolution

CtBP/BARS: a dual-function protein involved in transcription corepression and Golgi membrane fission

Released:
DOI: 10.2210/pdb1hku/pdb
PDB entry 1hku coloured by chain, front view.
PDB entry 1hku coloured by chain, side view.
PDB entry 1hku coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission.
Nardini M, Spanò S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M
EMBO J 22 3122-30 (2003)
PMID: 12805226

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-terminal-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 39.53 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Z2F5 (Residues: 1-350; Coverage: 81%)
Gene names: Bars, Ctbp1, Ctbp3
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand NAD 1 x NAD
Ligand GOL 1 x GOL
Ligand FMT 2 x FMT
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6422
Unit cell:
a: 88.705Å b: 88.705Å c: 163.014Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.281
Expression system: Escherichia coli BL21(DE3)

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Citations

22 review citations

Mechanism of leaf-shape determination.
Tsukaya H. (2006)
21 more

10 mentions without citation

CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission.
Nardini et al. (2003)
9 more