› 1hkv

X-ray diffraction
2.6Å resolution

mycobacterium diaminopimelate dicarboxylase (lysa)

Released:
DOI: 10.2210/pdb1hkv/pdb
PDB entry 1hkv coloured by chain, front view.
PDB entry 1hkv coloured by chain, side view.
PDB entry 1hkv coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of Mycobacterium tuberculosis diaminopimelate decarboxylase, an essential enzyme in bacterial lysine biosynthesis.
Gokulan K, Rupp B, Pavelka MS, Jacobs WR, Sacchettini JC
J Biol Chem 278 18588-96 (2003)
PMID: 12637582

Function and Biology Details

Reaction catalysed: 
Meso-2,6-diaminoheptanedioate = L-lysine + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diaminopimelate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 453 amino acids
Theoretical weight: 48.34 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIU7 (Residues: 1-447; Coverage: 100%)
Gene names: MTCY373.13, Rv1293, lysA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand LYS 2 x LYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P41212
Unit cell:
a: 111.452Å b: 111.452Å c: 238.253Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.268
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The aspartic acid metabolic pathway, an exciting and essential pathway in plants.
Azevedo et al. (2006)
5 more

6 mentions without citation

X-ray structure of Paramecium bursaria Chlorella virus arginine decarboxylase: insight into the structural basis for substrate specificity.
Shah et al. (2007)
5 more