› 1hmy

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE HHAI DNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYL-L-METHIONINE

Released:
DOI: 10.2210/pdb1hmy/pdb
PDB entry 1hmy coloured by chain, front view.
PDB entry 1hmy coloured by chain, side view.
PDB entry 1hmy coloured by chain, top view.
Source organism: Haemophilus haemolyticus
Primary publication:
Crystal structure of the HhaI DNA methyltransferase complexed with S-adenosyl-L-methionine.
Cheng X, Kumar S, Posfai J, Pflugrath JW, Roberts RJ
Cell 74 299-307 (1993)
PMID: 8343957

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II methyltransferase M.HhaI Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 37.04 KDa
Source organism: Haemophilus haemolyticus
Expression system: Not provided
UniProt:
  • Canonical: P05102 (Residues: 1-327; Coverage: 100%)
Gene name: hhaIM
Sequence domains: C-5 cytosine-specific DNA methylase
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 55.3Å b: 72.7Å c: 91Å
α: 90° β: 102.5° γ: 90°
R-values:
R R work R free
0.2 0.2 not available
Expression system: Not provided

Quick links

Citations

42 review citations

Eukaryotic cytosine methyltransferases.
Goll et al. (2005)
41 more

12 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
11 more