› 1hq0

X-ray diffraction
1.83Å resolution

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF E.COLI CYTOTOXIC NECROTIZING FACTOR TYPE 1

Released:
DOI: 10.2210/pdb1hq0/pdb
PDB entry 1hq0 coloured by chain, front view.
PDB entry 1hq0 coloured by chain, side view.
PDB entry 1hq0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the Rho-activating domain of Escherichia coli cytotoxic necrotizing factor 1.
Buetow L, Flatau G, Chiu K, Boquet P, Ghosh P
Nat Struct Biol 8 584-8 (2001)
PMID: 11427886

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytotoxic necrotizing factor 1 Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 32.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47106 (Residues: 720-1014; Coverage: 29%)
Gene name: cnf1
Sequence domains: Rho-activating domain of cytotoxic necrotizing factor
Structure domains: Cytotoxic necrotizing factor, Rho-activating domain

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, SSRL BEAMLINE BL1-5
Spacegroup: P65
Unit cell:
a: 55Å b: 55Å c: 176.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

Bacterial protein toxins that modify host regulatory GTPases.
Aktories K. (2011)
14 more

16 mentions without citation

Recent insights into Pasteurella multocida toxin and other G-protein-modulating bacterial toxins.
Wilson et al. (2010)
15 more