› 1hq1

X-ray diffraction
1.52Å resolution

STRUCTURAL AND ENERGETIC ANALYSIS OF RNA RECOGNITION BY A UNIVERSALLY CONSERVED PROTEIN FROM THE SIGNAL RECOGNITION PARTICLE

Released:
DOI: 10.2210/pdb1hq1/pdb
PDB entry 1hq1 coloured by chain, front view.
PDB entry 1hq1 coloured by chain, side view.
PDB entry 1hq1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and energetic analysis of RNA recognition by a universally conserved protein from the signal recognition particle.
Batey RT, Sagar MB, Doudna JA
J Mol Biol 307 229-46 (2001)
PMID: 11243816

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142811 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 12.04 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGD7 (Residues: 328-432; Coverage: 23%)
Gene names: JW5414, b2610, ffh
Sequence domains: Signal peptide binding domain
Structure domains: Signal recognition particle, SRP54 subunit, M-domain
4.5S RNA DOMAIN IV Chain: B
Molecule details ›
Chain: B
Length: 49 nucleotides
Theoretical weight: 15.93 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand K 3 x K
1 modified residue:
Ligand CCC 1 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 136.555Å b: 78.337Å c: 32.872Å
α: 90° β: 96.24° γ: 90°
R-values:
R R work R free
0.157 0.151 0.199
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
8 more

23 mentions without citation

The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches.
Gerovac et al. (2021)
22 more