› 1hsj

X-ray diffraction
2.3Å resolution

SARR MBP FUSION STRUCTURE

Released:
DOI: 10.2210/pdb1hsj/pdb
PDB entry 1hsj coloured by chain, front view.
PDB entry 1hsj coloured by chain, side view.
PDB entry 1hsj coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the SarR protein from Staphylococcus aureus.
Liu Y, Manna A, Li R, Martin WE, Murphy RC, Cheung AL, Zhang G
Proc Natl Acad Sci U S A 98 6877-82 (2001)
PMID: 11381122

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator SarR; Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 487 amino acids
Theoretical weight: 54.43 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-387; Coverage: 98%)
  • Canonical: Q9F0R1 (Residues: 1-115; Coverage: 100%)
Gene names: JW3994, SAOUHSC_02566, b4034, malE, sarR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GLC 4 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 64.739Å b: 70.639Å c: 75.473Å
α: 65.72° β: 67.18° γ: 69.58°
R-values:
R R work R free
0.263 0.263 0.304
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Regulation of virulence determinants in vitro and in vivo in Staphylococcus aureus.
Cheung et al. (2004)
8 more

15 mentions without citation

A synergistic approach to protein crystallization: combination of a fixed-arm carrier with surface entropy reduction.
Moon et al. (2010)
14 more