› 1hv5

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF THE STROMELYSIN-3 (MMP-11) CATALYTIC DOMAIN COMPLEXED WITH A PHOSPHINIC INHIBITOR

Released:
DOI: 10.2210/pdb1hv5/pdb
PDB entry 1hv5 coloured by chain, front view.
PDB entry 1hv5 coloured by chain, side view.
PDB entry 1hv5 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the stromelysin-3 (MMP-11) catalytic domain complexed with a phosphinic inhibitor mimicking the transition-state.
Gall AL, Ruff M, Kannan R, Cuniasse P, Yiotakis A, Dive V, Rio MC, Basset P, Moras D
J Mol Biol 307 577-86 (2001)
PMID: 11254383

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stromelysin-3 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 165 amino acids
Theoretical weight: 19.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q02853 (Residues: 102-265; Coverage: 36%)
Gene name: Mmp11
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

4 bound ligands:
Ligand ZN 12 x ZN
Ligand CA 6 x CA
Ligand CPS 12 x CPS
Ligand RXP 6 x RXP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 140.1Å b: 148.5Å c: 91.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.262
Expression system: Escherichia coli BL21

Quick links

Citations

16 review citations

Biochemical and Biological Attributes of Matrix Metalloproteinases.
Cui et al. (2017)
15 more

10 mentions without citation

Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures.
Wlodawer et al. (2008)
9 more