› 1hw6

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF APO-2,5-DIKETO-D-GLUCONATE REDUCTASE

Released:
DOI: 10.2210/pdb1hw6/pdb
PDB entry 1hw6 coloured by chain, front view.
PDB entry 1hw6 coloured by chain, side view.
PDB entry 1hw6 coloured by chain, top view.
Source organism: Corynebacterium sp.
Primary publication:
Structural assembly of the active site in an aldo-keto reductase by NADPH cofactor.
Sanli G, Blaber M
J Mol Biol 309 1209-18 (2001)
PMID: 11399090

Function and Biology Details

Reaction catalysed: 
2-dehydro-L-gulonate + NADP(+) = 2,5-didehydro-D-gluconate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,5-diketo-D-gluconic acid reductase A Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 30.15 KDa
Source organism: Corynebacterium sp.
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06632 (Residues: 1-278; Coverage: 100%)
Gene name: dkgA
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 53.03Å b: 53.94Å c: 89.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 not available not available
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Extreme makeover: Engineering the activity of a thermostable alcohol dehydrogenase (AdhD) from Pyrococcus furiosus.
Solanki et al. (2016)
1 more

6 mentions without citation

Identification of a novel NADH-specific aldo-keto reductase using sequence and structural homologies.
Di Luccio et al. (2006)
5 more