› 1hx3

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF E.COLI ISOPENTENYL DIPHOSPHATE:DIMETHYLALLYL DIPHOSPHATE ISOMERASE

Released:
DOI: 10.2210/pdb1hx3/pdb
PDB entry 1hx3 coloured by chain, front view.
PDB entry 1hx3 coloured by chain, side view.
PDB entry 1hx3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of isopentenyl diphosphate:dimethylallyl diphosphate isomerase.
Durbecq V, Sainz G, Oudjama Y, Clantin B, Bompard-Gilles C, Tricot C, Caillet J, Stalon V, Droogmans L, Villeret V
EMBO J 20 1530-7 (2001)
PMID: 11285217

Function and Biology Details

Reaction catalysed: 
Isopentenyl diphosphate = prenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl-diphosphate Delta-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q46822 (Residues: 1-182; Coverage: 100%)
Gene names: JW2857, b2889, idi, ygfV
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand MN 2 x MN
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P212121
Unit cell:
a: 69.303Å b: 72.621Å c: 92.461Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.249
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
11 more

2 mentions without citation

Crystal structure of isopentenyl diphosphate:dimethylallyl diphosphate isomerase.
Durbecq et al. (2001)
1 more