› 1hx8

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF N-TERMINAL DOMAIN OF DROSOPHILA AP180

Released:
DOI: 10.2210/pdb1hx8/pdb
PDB entry 1hx8 coloured by chain, front view.
PDB entry 1hx8 coloured by chain, side view.
PDB entry 1hx8 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
A novel all helix fold of the AP180 amino-terminal domain for phosphoinositide binding and clathrin assembly in synaptic vesicle endocytosis.
Mao Y, Chen J, Maynard JA, Zhang B, Quiocho FA
Cell 104 433-40 (2001)
PMID: 11239400

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol-binding clathrin assembly protein LAP Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 33.65 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9VI75 (Residues: 1-299; Coverage: 64%)
Gene names: CG2520, lap
Sequence domains: ANTH domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 105.632Å b: 106.933Å c: 79.207Å
α: 90° β: 119.3° γ: 90°
R-values:
R R work R free
0.221 0.211 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

22 review citations

Biological basket weaving: formation and function of clathrin-coated vesicles.
Brodsky et al. (2001)
21 more

2 mentions without citation

Endocytic adaptors--social networking at the plasma membrane.
Reider et al. (2011)
1 more