› 1hy0

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF WILD TYPE DUCK DELTA 1 CRYSTALLIN (EYE LENS PROTEIN)

Released:
DOI: 10.2210/pdb1hy0/pdb
PDB entry 1hy0 coloured by chain, front view.
PDB entry 1hy0 coloured by chain, side view.
PDB entry 1hy0 coloured by chain, top view.
Source organism: Anas platyrhynchos
Primary publication:
Structural studies of duck delta 1 and delta 2 crystallin suggest conformational changes occur during catalysis.
Sampaleanu LM, Vallée F, Slingsby C, Howell PL
Biochemistry 40 2732-42 (2001)
PMID: 11258884

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-1 crystallin Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 51.21 KDa
Source organism: Anas platyrhynchos
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P24057 (Residues: 1-466; Coverage: 100%)
Gene name: ASL1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P3121
Unit cell:
a: 100.6Å b: 100.6Å c: 168Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.19 0.233
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Moonlighting proteins--an update.
Jeffery CJ. (2009)
2 more

2 mentions without citation

Structural studies of duck delta2 crystallin mutants provide insight into the role of Thr161 and the 280s loop in catalysis.
Sampaleanu et al. (2004)
1 more