› 1hzo

X-ray diffraction
1.75Å resolution

STRUCTURE OF CLASS A CEPHALOSPORINASE FROM PROTEUS VULGARIS K1

Released:
DOI: 10.2210/pdb1hzo/pdb
Model geometry
Fit model/data
PDB entry 1hzo coloured by chain, front view.
PDB entry 1hzo coloured by chain, side view.
PDB entry 1hzo coloured by chain, top view.
Source organism: Proteus vulgaris
Primary publication:
Structure of an extended-spectrum class A beta-lactamase from Proteus vulgaris K1.
Nukaga M, Mayama K, Crichlow GV, Knox JR
J Mol Biol 317 109-17 (2002)
PMID: 11916382

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 29.69 KDa
Source organism: Proteus vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P52664 (Residues: 30-300; Coverage: 100%)
Gene names: blaB, cumA
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 58.65Å b: 66.15Å c: 134.394Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.193
Expression system: Escherichia coli

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Citations

1 review citation

Global dissemination of beta-lactamases mediating resistance to cephalosporins and carbapenems.
Gootz TD. (2004)
5 other articles

6 mentions without citation

Crystal structure and activity studies of the Mycobacterium tuberculosis beta-lactamase reveal its critical role in resistance to beta-lactam antibiotics.
Wang et al. (2006)
5 more