› 1hzt

X-ray diffraction
1.45Å resolution

CRYSTAL STRUCTURE OF METAL-FREE ISOPENTENYL DIPHOSPHATE:DIMETHYLALLYL DIPHOSPHATE ISOMERASE

Released:
DOI: 10.2210/pdb1hzt/pdb
PDB entry 1hzt coloured by chain, front view.
PDB entry 1hzt coloured by chain, side view.
PDB entry 1hzt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of isopentenyl diphosphate:dimethylallyl diphosphate isomerase.
Durbecq V, Sainz G, Oudjama Y, Clantin B, Bompard-Gilles C, Tricot C, Caillet J, Stalon V, Droogmans L, Villeret V
EMBO J 20 1530-7 (2001)
PMID: 11285217

Function and Biology Details

Reaction catalysed: 
Isopentenyl diphosphate = prenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl-diphosphate Delta-isomerase Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q46822 (Residues: 1-182; Coverage: 100%)
Gene names: JW2857, b2889, idi, ygfV
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P3221
Unit cell:
a: 71.4Å b: 71.4Å c: 61.85Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
11 more

6 mentions without citation

Crystal structure of isopentenyl diphosphate:dimethylallyl diphosphate isomerase.
Durbecq et al. (2001)
5 more