› 1i0g

X-ray diffraction
1.45Å resolution

1.45 A STRUCTURE OF THE A-DECAMER GCGTATACGC WITH A SINGLE 2'-O-FLUOROETHYL THYMINE IN PLACE OF T6, MEDIUM NA-SALT

Released:
DOI: 10.2210/pdb1i0g/pdb
PDB entry 1i0g coloured by chain, front view.
PDB entry 1i0g coloured by chain, side view.
PDB entry 1i0g coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Detection of alkali metal ions in DNA crystals using state-of-the-art X-ray diffraction experiments.
Tereshko V, Wilds CJ, Minasov G, Prakash TP, Maier MA, Howard A, Wawrzak Z, Manoharan M, Egli M
Nucleic Acids Res 29 1208-15 (2001)
PMID: 11222771

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113927 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*GP*CP*GP*TP*AP*(125)P*AP*CP*GP*C)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 10 nucleotides
Theoretical weight: 3.11 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
1 modified residue:
Ligand 125 2 x 125

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 24.96Å b: 43.57Å c: 45.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.19
Expression system: Not provided

Quick links

Citations

11 review citations

Ions and RNA folding.
Draper et al. (2005)
10 more

1 mention without citation

Detection of alkali metal ions in DNA crystals using state-of-the-art X-ray diffraction experiments.
Tereshko et al. (2001)