› 1i0o

X-ray diffraction
2Å resolution

1.6 A STRUCTURE OF THE A-DECAMER GCGTATACGC WITH A SINGLE 2'-O-METHYL-3'-METHYLENEPHOSPHONATE THYMINE IN PLACE OF T6, HIGH K-SALT

Released:
DOI: 10.2210/pdb1i0o/pdb
PDB entry 1i0o coloured by chain, front view.
PDB entry 1i0o coloured by chain, side view.
PDB entry 1i0o coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Detection of alkali metal ions in DNA crystals using state-of-the-art X-ray diffraction experiments.
Tereshko V, Wilds CJ, Minasov G, Prakash TP, Maier MA, Howard A, Wawrzak Z, Manoharan M, Egli M
Nucleic Acids Res 29 1208-15 (2001)
PMID: 11222771

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113932 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*GP*CP*GP*TP*AP*(T23)P*AP*CP*GP*C)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 10 nucleotides
Theoretical weight: 3.07 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 24.65Å b: 44.61Å c: 44.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.246
Expression system: Not provided

Quick links

Citations

11 review citations

Ions and RNA folding.
Draper et al. (2005)
10 more

2 mentions without citation

Detection of alkali metal ions in DNA crystals using state-of-the-art X-ray diffraction experiments.
Tereshko et al. (2001)
1 more