› 1i2w

X-ray diffraction
1.7Å resolution

BETA-LACTAMASE FROM BACILLUS LICHENIFORMIS BS3 COMPLEXED WITH CEFOXITIN

Released:
DOI: 10.2210/pdb1i2w/pdb
PDB entry 1i2w coloured by chain, front view.
PDB entry 1i2w coloured by chain, side view.
PDB entry 1i2w coloured by chain, top view.
Source organism: Bacillus licheniformis
Primary publication:
Crystal structures of the Bacillus licheniformis BS3 class A beta-lactamase and of the acyl-enzyme adduct formed with cefoxitin.
Fonzé E, Vanhove M, Dive G, Sauvage E, Frère JM, Charlier P
Biochemistry 41 1877-85 (2002)
PMID: 11827533

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 31.29 KDa
Source organism: Bacillus licheniformis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00808 (Residues: 26-307; Coverage: 100%)
Gene names: blaP, penP
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand 1QL 2 x 1QL
Ligand OUT 1 x OUT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE D41A
Spacegroup: P21
Unit cell:
a: 47.344Å b: 106.372Å c: 63.873Å
α: 90° β: 94.18° γ: 90°
R-values:
R R work R free
0.216 0.216 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Class A carbapenemases.
Walther-Rasmussen et al. (2007)
1 more

6 mentions without citation

Crystal structure and activity studies of the Mycobacterium tuberculosis beta-lactamase reveal its critical role in resistance to beta-lactam antibiotics.
Wang et al. (2006)
5 more