› 1i31

X-ray diffraction
2.5Å resolution

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 CLATHRIN ADAPTOR, COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM AT 2.5 A RESOLUTION

Released:
DOI: 10.2210/pdb1i31/pdb
PDB entry 1i31 coloured by chain, front view.
PDB entry 1i31 coloured by chain, side view.
PDB entry 1i31 coloured by chain, top view.
Source organism: Rattus norvegicus
Entry authors: Modis Y, Boll W, Rapoport I, Kirchhausen T

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108683 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AP-2 complex subunit mu Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 35.75 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84092 (Residues: 122-435; Coverage: 72%)
Gene name: Ap2m1
Sequence domains: Adaptor complexes medium subunit family
Structure domains: Mu homology domain, subdomain B
receptor protein-tyrosine kinase Chain: P
Molecule details ›
Chain: P
Length: 6 amino acids
Theoretical weight: 801 Da
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: A8IP97 (Residues: 139-144; Coverage: 3%)
Gene names: Egfr, egfr

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P64
Unit cell:
a: 125.727Å b: 125.727Å c: 74.634Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.247 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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