› 1i3a

X-ray diffraction
2.15Å resolution

RNASE HII FROM ARCHAEOGLOBUS FULGIDUS WITH COBALT HEXAMMINE CHLORIDE

Released:
DOI: 10.2210/pdb1i3a/pdb
PDB entry 1i3a coloured by chain, front view.
PDB entry 1i3a coloured by chain, side view.
PDB entry 1i3a coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structural biochemistry of a type 2 RNase H: RNA primer recognition and removal during DNA replication.
Chapados BR, Chai Q, Hosfield DJ, Qiu J, Shen B, Tainer JA
J Mol Biol 307 541-56 (2001)
PMID: 11254381

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HII Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.39 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29634 (Residues: 1-205; Coverage: 100%)
Gene names: AF_0621, rnhB
Sequence domains: Ribonuclease HII
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NCO 1 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6522
Unit cell:
a: 74.555Å b: 74.555Å c: 139.684Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
Tadokoro et al. (2009)
7 more

1 mention without citation

A new definition and properties of the similarity value between two protein structures.
Saberi Fathi SM. (2016)